<p>[[{"type":"media","view_mode":"media_large","fid":"1375","attributes":{"alt":"nvista microscope.jpg","class":"media-image","height":"316","title":"","width":"363"}}]]</p> <ul> <li><a href="#specs"><strong><span>Product Specifications</span></strong></a></li> <li><strong><span><a href="#caremaintenancevid">Care and Maintenance</a> (Video Tutorial)<a></a></span></strong></li> <li><a href="#Cleaning%20Microscope"><strong><span>Cleaning the Microscope</span></strong></a></li> <li><strong><span><a href="#tips">Tips and Tricks </a>(Video Tutorial)</span></strong></li> </ul> <p><span><strong>V2 Microscope Specifications:</strong></span></p> <ul> <li><strong>Mass:</strong><span> 2 grams</span></li> <li><strong>Dimensions:</strong><span> 11 mm <span>×</span> 14 mm <span>×</span> 20 mm</span></li> <li><strong>Modality:</strong><span> Single-channel epifluorescence</span></li> <li><strong>Excitation:</strong><span> 475/10 nm (blue)</span></li> <li><strong>Collection:</strong><span> 535/50 nm (green)</span></li> <li><span><strong>Focus:</strong> nominal focus of ~200 <span>µ</span>m (<span>micrometers)</span> below objective lens, with ~<span>100 <span>µ</span>m of adjustment above or below this plane </span>available through the focusing element on the microscope tower. (Learn how to adjust the focus in the video tutorial on this page.)</span></li> <li><span><strong>LED Power:</strong> 2 mW/mm²a</span>t 100%.  (Note: <span> The LED power output is linear from 0-2mW; t<span>he software LED percentage is a linear scale from 0% to 100%.</span>)</span></li> </ul> <p><strong>Integrated HD Camera Specifications:</strong></p> <ul> <li><strong>Maximum Field of View:</strong><span> 1440 pixels x 1080 pixels; 900 <span>µ</span>m x 650 <span>µ</span>m</span></li> <li><strong>Temporal Resolution:</strong><span> 25 fps* at full field of view</span></li> </ul> <p><span>*Maximum temporal resolution of microscope dependent on computer system.</span></p> <p><span><strong><a></a>Care and Maintenance</strong></span></p> <p></p> <p>You<span><span></span></span><span>r nVista HD microscope is a robust piece of imaging equipment, designed for multiple imaging sessions and reliable operation.  However, it is important to remember that it also contains delicate components that require gentle handling.  </span><span>Preventative care and maintenance </span><span>will help extend the life of your system, as well as prevent disruptions to your experiments.</span></p> <p><span></span>Keep the nVista microscope in the provided plastic case, and always store in and utilize the foam carrier when not in use.  Never let the microscope housing, wires or lens come into contact with liquids or adhesives.  Prevent hard impacts. </p> <p>Take special care of the <span>microscope data cable</span>, as this is how all of your data is carried from your animal to your computer.  Do not allow animals to gain access to the cable, especially when they are left unattended during behavioral paradigms. </p> <h3><strong><a></a>Cleaning the Microscope</strong></h3> <p>Do not immerse the microscope in liquids.  To clean the microscope objective imaging surface, check the surface by examining the lens surface under a bright light.  Use lens paper, slightly wet it with 70% ethanol, and gently rub the imaging surface clean.  For debris stuck to the lens, use forceps wrapped in moist lens paper to <strong>gently</strong> scrape the object off the imaging surface.  </p> <ul> <li>During cleaning, take care not to scratch the lens surface, and <strong>be careful not to let any ethanol drip or seep into the cracks around the objective into the body of the microscope.</strong></li> </ul> <p></p> <p><span><strong><a></a>Tips and Tricks</strong></span></p> <p><strong>Adjust the Microscope Focus</strong></p> <p>Learn how to adjust the focus of your microscope with the video demonstration below.  If you are imaging through the cannula glass in a deep-brain prep, keep in mind that the cannula glass is approximately 100 <span>µ</span>m thick, leaving your plane of focus within the tissue 200 <span>µ</span>m <span>±</span> 100 <span>µ</span>m below the cannula lens.</p> <p></p>